Difference between revisions of "Part:BBa K4160016"
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| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><h3>Sequence and Features</h3></span> |
<partinfo>BBa_K4160016 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4160016 SequenceAndFeatures</partinfo> | ||
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<h2>Usage & biology</h2> | <h2>Usage & biology</h2> | ||
| − | <a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022</a> used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.<sup>1</sup> Upon activation of the GEMS receptor, STAT-induced SEAP will be expressed and secreted via the JAK/STAT signaling pathway. In the project of <a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022</a> | + | <a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022</a> used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.<sup>1</sup> Upon activation of the GEMS receptor, STAT-induced SEAP will be expressed and secreted via the JAK/STAT signaling pathway. In the project of <a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022,</a> SEAP was used as a genetic reporter protein to investigate the activation of the GEMS receptor (<a href="https://parts.igem.org/Part:BBa_K4160008">BBa_K4160008</a>, <a href="https://parts.igem.org/Part:BBa_K4160009">BBa_K4160009</a>, <a href="https://parts.igem.org/Part:BBa_K4160010">BBa_K4160010</a>, <a href="https://parts.igem.org/Part:BBa_K4160012">BBa_K4160012</a>, and <a href="https://parts.igem.org/Part:BBa_K4160013">BBa_K4160013</a>). STAT-induced SEAP was expressed in a pLS13 plasmid.</p><br> |
<h2>Characterization </h2> | <h2>Characterization </h2> | ||
Latest revision as of 14:18, 12 October 2022
STAT-induced SEAP
This composite part encodes for STAT-induced SEAP and is composed of the basic parts STAT promotor (BBa_K4160006) and SEAP (BBa_K1470004).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 209
Illegal XbaI site found at 233
Illegal PstI site found at 53 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 209
Illegal PstI site found at 53 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 209
Illegal BamHI site found at 227
Illegal BamHI site found at 459 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 209
Illegal XbaI site found at 233
Illegal PstI site found at 53 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 209
Illegal XbaI site found at 233
Illegal PstI site found at 53
Illegal NgoMIV site found at 770
Illegal NgoMIV site found at 1739 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1100
Illegal BsaI.rc site found at 1589
Usage & biology
TU-Eindhoven 2022 used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.1 Upon activation of the GEMS receptor, STAT-induced SEAP will be expressed and secreted via the JAK/STAT signaling pathway. In the project of TU-Eindhoven 2022, SEAP was used as a genetic reporter protein to investigate the activation of the GEMS receptor (BBa_K4160008, BBa_K4160009, BBa_K4160010, BBa_K4160012, and BBa_K4160013). STAT-induced SEAP was expressed in a pLS13 plasmid.Characterization
Characterization of STAT-induced SEAP expression by the GEMS system can be found on the BBa_K4160008 page.
References