Difference between revisions of "Part:BBa K4229003"
 
(4 intermediate revisions by 2 users not shown)
Line 4: Line 4:
  
  
−
TnaB is involved in the transport of L-tryptophan through the cell membrane. Here we overexpressed it improve the L-tryptophan intake of this amino acid.
+
The low affinity tryptophan permease TnaB is involved in the transport of tryptophan across the cytoplasmic membrane. It is a multi-pass membrane protein.
  
−
[[File:Grafik indigo weier bg.png|900px|thumb|left|]]
+
As one part of our toolbox to produce indigo/indirubin, we express TnaB to improve the cellular uptake of L-tryptophan.
−
<b>Figure 1: Schematic representation of the indigo/indirubin pathway.</b> L-Tryptophan is imported by the membrane protein TnaB (low affinity tryptophan permease). L-tryptophan is cleaved into indole, NH4+ and pyruvate by the Tryptophanase TnaA. The reaction continues by the hydroxylation of indole through XiaI. To enhance the effectivity of this enzyme, the NAD(P)H-flavin reductase provides XiaI with FADH2 by adding Hydrogen to FAD. Finally, indole is transformed to either 3-Hydroxyindole or 2-Hydroxyindole. These two substances spontaneously react to 3-Oxindole and 2-Oxindole through the secession of hydrogen from the OH-group. Through spontaneous dimerization indigo and indirubin are formed. Graphic adapted from [1].
+
 
 +
[[File:Grafik indigo weier bg.png|900px|thumb|left|
 +
<b>Figure 1: Schematic representation of the indigo/indirubin pathway.</b> L-Tryptophan is imported by the membrane protein TnaB (low affinity tryptophan permease). L-tryptophan is cleaved into indole, NH4+ and pyruvate by the Tryptophanase TnaA. The reaction continues by the hydroxylation of indole through XiaI. To enhance the effectivity of this enzyme, the NAD(P)H-flavin reductase provides XiaI with FADH2 by adding Hydrogen to FAD. Finally, indole is transformed to either 3-Hydroxyindole or 2-Hydroxyindole. These two substances spontaneously react to 3-Oxindole and 2-Oxindole through the secession of hydrogen from the OH-group. Through spontaneous dimerization indigo and indirubin are formed. Graphic adapted from [1].]]
 
<br>
 
<br>
 
<br>
 
<br>
  
−
References:<br>
+
<b>References:</b>
 +
<br>
 
[1] H. Yin et al., “Efficient Bioproduction of Indigo and Indirubin by Optimizing a Novel Terpenoid Cyclase XiaI in Escherichia coli,” ACS Omega, vol. 6, no. 31, pp. 20569–20576, 2021, doi: 10.1021/acsomega.1c02679.
 
[1] H. Yin et al., “Efficient Bioproduction of Indigo and Indirubin by Optimizing a Novel Terpenoid Cyclase XiaI in Escherichia coli,” ACS Omega, vol. 6, no. 31, pp. 20569–20576, 2021, doi: 10.1021/acsomega.1c02679.
  

Latest revision as of 11:10, 12 October 2022


TnaB tryptophan transport protein


The low affinity tryptophan permease TnaB is involved in the transport of tryptophan across the cytoplasmic membrane. It is a multi-pass membrane protein.

As one part of our toolbox to produce indigo/indirubin, we express TnaB to improve the cellular uptake of L-tryptophan.

Figure 1: Schematic representation of the indigo/indirubin pathway. L-Tryptophan is imported by the membrane protein TnaB (low affinity tryptophan permease). L-tryptophan is cleaved into indole, NH4+ and pyruvate by the Tryptophanase TnaA. The reaction continues by the hydroxylation of indole through XiaI. To enhance the effectivity of this enzyme, the NAD(P)H-flavin reductase provides XiaI with FADH2 by adding Hydrogen to FAD. Finally, indole is transformed to either 3-Hydroxyindole or 2-Hydroxyindole. These two substances spontaneously react to 3-Oxindole and 2-Oxindole through the secession of hydrogen from the OH-group. Through spontaneous dimerization indigo and indirubin are formed. Graphic adapted from [1].



References:
[1] H. Yin et al., “Efficient Bioproduction of Indigo and Indirubin by Optimizing a Novel Terpenoid Cyclase XiaI in Escherichia coli,” ACS Omega, vol. 6, no. 31, pp. 20569–20576, 2021, doi: 10.1021/acsomega.1c02679.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]