Difference between revisions of "Part:BBa K4165123"
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Latest revision as of 09:19, 13 October 2022
HTRA Binding Peptide 29
part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
Usage and Biology
Synthetic peptide acquired by Phage display, Binds specifically to the water-sites of PDZ domain of HTRA1 Protein with IC50 of 1.8 ± 0.1 and ΔG of 90 kcal/mol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Dry lab characterizations
Modeling
Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
| cbeta_deviations | clashscore | molprobity | ramachandran_favored | ramachandran_outliers | Qmean_4 | Qmean_6 |
|---|---|---|---|---|---|---|
| 0 | 0 | 2.87 | 0 | 0 | -6.45183 | -5.513969 |
Figure 1.: 3D Model of H2C peptide Modelled by AlphaFold2.0 displayed in Pymol.
References
Beuming, T., Farid, R., & Sherman, W. (2009). High‐energy water sites determine peptide binding affinity and specificity of PDZ domains. Protein Science, 18(8), 1609-1619.