Difference between revisions of "Part:BBa K4165108"

Latest revision as of 09:43, 13 October 2022

HTRA Binding Peptide 14

Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Decameric peptide, Binds specifically to PDZ domain of HTRA1 Protein with ΔG of 3.8 ± 0.2 kcal/mol.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

Modeling

Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations	clashscore	molprobity	ramachandran_favored	ramachandran_outliers	Qmean_4	Qmean_6
0	0	1.62	100	0	-5.843485/td>	-4.746417

                      Figure 1.: 3D Model of H1C peptide Modelled by AlphaFold2.0 displayed in Pymol.

References

Rey, J., Breiden, M., Lux, V., Bluemke, A., Steindel, M., Ripkens, K., ... & Ehrmann, M. (2022). An allosteric HTRA1-calpain 2 complex with restricted activation profile. Proceedings of the National Academy of Sciences, 119(14), e2113520119.

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 Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
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-<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1n.jpg" style="margin-left:200px;" alt="" width="400" /></p>
+<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1n.jpg" style="margin-left:250px;" alt="" width="400" /></p>
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-                  Figure 1.: 3D Model of H1C peptide Modelled by AlphaFold2.0 displayed in Pymol.
+                       Figure 1.: 3D Model of H1C peptide Modelled by AlphaFold2.0 displayed in Pymol.
 ===References===