Difference between revisions of "Part:BBa K175046:Design"
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<partinfo>BBa_K175046 short</partinfo> | <partinfo>BBa_K175046 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | This part was designed to express RFP when it is placed inside ''E. coli''. If this part is transformed together with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K175047 BBa_K175047] the result is a delay in the expression of GFP after IPTG induction. | |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
+ | This part was [http://2009.igem.org/Team:TUDelft/Synthetic_Transcriptional_Cascade:_The_plan designed] by the TUDelft 09 team. The design was based on the following works. | ||
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+ | Hooshangi S, Thiberge S, Weiss R, 2005, [http://www.pnas.org/content/102/10/3581.abstract Ultrasensitivity and noise propagation in a synthetic transcriptional cascade], PNAS, 102,10 :3581–3586. | ||
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+ | Rosenfeld N and Alon U, [http://www.ncbi.nlm.nih.gov/pubmed/12787666 Response Delays and Structure of Transcription Networks], J. Mol. Biol (2003) 329, 645-654. |
Latest revision as of 19:58, 21 October 2009
Plasmid 1 for Transcriptional Cascade subproject TU Delft iGEM 09
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1436
Illegal AgeI site found at 1548 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was designed to express RFP when it is placed inside E. coli. If this part is transformed together with BBa_K175047 the result is a delay in the expression of GFP after IPTG induction.
Source
This part was constructed using biobricks from the registry
References
This part was [http://2009.igem.org/Team:TUDelft/Synthetic_Transcriptional_Cascade:_The_plan designed] by the TUDelft 09 team. The design was based on the following works.
Hooshangi S, Thiberge S, Weiss R, 2005, [http://www.pnas.org/content/102/10/3581.abstract Ultrasensitivity and noise propagation in a synthetic transcriptional cascade], PNAS, 102,10 :3581–3586.
Rosenfeld N and Alon U, [http://www.ncbi.nlm.nih.gov/pubmed/12787666 Response Delays and Structure of Transcription Networks], J. Mol. Biol (2003) 329, 645-654.