Difference between revisions of "Part:BBa K175046"
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− | This part is the plasmid 1 of the transcriptional cascade subproject of TU Delft iGEM. It generates the transcriptional regulator cI and RFP under the control of PTet promoter. This part in combination with the plasmid 2 (K175047) will lead to a delay in the expression of GFP after IPTG induction | + | This part is the plasmid 1 of the transcriptional cascade subproject of TU Delft iGEM. It generates the transcriptional regulator cI and RFP under the control of PTet promoter. This part in combination with the plasmid 2 (K175047) will lead to a delay in the expression of GFP after IPTG induction. The detailed description of the behavior and characterization of this part can be seen [http://2009.igem.org/Team:TUDelft/Results here]. And the characterization of the delay device can be seen [https://parts.igem.org/Part:BBa_K175046:Experience here]. |
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Latest revision as of 04:52, 1 November 2009
Plasmid 1 for Transcriptional Cascade subproject TU Delft iGEM 09
This part is the plasmid 1 of the transcriptional cascade subproject of TU Delft iGEM. It generates the transcriptional regulator cI and RFP under the control of PTet promoter. This part in combination with the plasmid 2 (K175047) will lead to a delay in the expression of GFP after IPTG induction. The detailed description of the behavior and characterization of this part can be seen [http://2009.igem.org/Team:TUDelft/Results here]. And the characterization of the delay device can be seen here.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1436
Illegal AgeI site found at 1548 - 1000COMPATIBLE WITH RFC[1000]