Difference between revisions of "Part:BBa K4165097"

(→‎Dry Lab Characterization)
 
(2 intermediate revisions by 2 users not shown)
Line 7: Line 7:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with ΔG of –8.2 kJ.mol<sup>-1</sup> and IC<sub>50</sub> of 2.5 ± 0.4
+
Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with ΔG of –8.2 kcal/mol and IC<sub>50</sub> of 2.5 ± 0.4
  
 
<!-- -->
 
<!-- -->
Line 15: Line 15:
  
 
===Dry Lab Characterization===
 
===Dry Lab Characterization===
 +
<p style=" font-weight: bold; font-size:14px;"> Modeling </p>
 
Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
 
Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
−
 
−
<p style=" font-weight: bold; font-size:14px;"> Modeling </p>
 
−
 
  
 
<html>
 
<html>
Line 50: Line 48:
 
</body>
 
</body>
 
</html>
 
</html>
 +
  
 
<html>
 
<html>

Latest revision as of 11:06, 13 October 2022


HTRA Binding Peptide 3

Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with ΔG of –8.2 kcal/mol and IC50 of 2.5 ± 0.4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Dry Lab Characterization

Modeling

Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations clashscore molprobity ramachandran_favored ramachandran_outliers Qmean_4 Qmean_6
0 129.77 4.76 25 75 -6.45183 -5.513969 



                 Figure 1.: 3D Model of H1C peptide Modelled by AlphaFold2.0 displayed in Pymol.

References

1.Romero-Molina, S., Ruiz-Blanco, Y. B., Mieres-Perez, J., Harms, M., Münch, J., Ehrmann, M., & Sanchez-Garcia, E. (2022). PPI-Affinity: A Web Tool for the Prediction and Optimization of Protein–Peptide and Protein–Protein Binding Affinity. Journal of Proteome Research. 2.Runyon, S. T., Zhang, Y., Appleton, B. A., Sazinsky, S. L., Wu, P., Pan, B., ... & Sidhu, S. S. (2007). Structural and functional analysis of the PDZ domains of human HtrA1 and HtrA3. Protein Science, 16(11), 2454-2471.