Difference between revisions of "Part:BBa K4367011:Design"

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===Design Notes===
 
===Design Notes===
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This is elaborated in the long description.
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This is elaborated in the long description. This part has been designed in accordance with the golden gate assembley method called MoClo. As such it contains associated MoClo overhangs. The 6xHis-tag is included for protein extraction.
  
 
===Source===
 
===Source===
  
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It consists of two flourescent proteins, mNeongreen and mRuby3, that are fused together with a cleavable linker. The linker is a typical GS-linker and Tobacco Etch Virus Protease is able to cleave a target sequence within the linker.
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mRuby3 is derived from Entacmaea quadricolor, and mNeongreen is derived from Branchiostoma lanceolatum.
  
 
===References===
 
===References===

Latest revision as of 11:58, 12 October 2022


FRET Protein (FP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1504
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 1511


Design Notes

This is elaborated in the long description. This part has been designed in accordance with the golden gate assembley method called MoClo. As such it contains associated MoClo overhangs. The 6xHis-tag is included for protein extraction.

Source

mRuby3 is derived from Entacmaea quadricolor, and mNeongreen is derived from Branchiostoma lanceolatum.

References