Difference between revisions of "Part:BBa K4488016"
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freeuse Green Fluorescent Protein-Y, a yellow shifted GFP that fluoresces under blue light. | freeuse Green Fluorescent Protein-Y, a yellow shifted GFP that fluoresces under blue light. | ||
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| + | This part is a variant of fuGFP (Part:BBa K3814004) which was originally designed to create a GFP variant not protected by patents and able to facilitate any kind of research. Using PCR with degenerate primers we introduced the S66G mutation in the fluorophore to create fuGFPy. The variant has a yellow-shifted excitation peak (figure 1). We have also created a fusion protein of fuGFPy connected to a cellulose-binding domain which allows it to be immobilised on cellulose (Part:BBa K4488019). | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | [[File:fuGFP_variants_spectra.png|centre|thumb|700px| Figure 1. Absorption and emission spectrum for fuGFPy and other fuGFP variants (-b,-a)]] | ||
Latest revision as of 22:38, 11 October 2022
fuGFPy (Yellow Shifted free-use GFP)
freeuse Green Fluorescent Protein-Y, a yellow shifted GFP that fluoresces under blue light.
This part is a variant of fuGFP (Part:BBa K3814004) which was originally designed to create a GFP variant not protected by patents and able to facilitate any kind of research. Using PCR with degenerate primers we introduced the S66G mutation in the fluorophore to create fuGFPy. The variant has a yellow-shifted excitation peak (figure 1). We have also created a fusion protein of fuGFPy connected to a cellulose-binding domain which allows it to be immobilised on cellulose (Part:BBa K4488019).
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 151
- 1000COMPATIBLE WITH RFC[1000]