Difference between revisions of "Part:BBa K4165114"
 
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<partinfo>BBa_K4165114 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165114 SequenceAndFeatures</partinfo>
 
   
 
   
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===Functional Parameters===
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===Dry lab characterizations===
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===Modeling===
 
Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
 
Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
  
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cbeta_deviations = 0, clashscore = 40.65, molprobity = 4.06, ramachandran_favored = 20, ramachandran_outliers = 60, Qmean_4 = -6.45183, Qmean6 = -5.51397
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table, th, td {
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    <th>cbeta_deviations</th>
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    <th>clashscore</th>
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    <th>molprobity</th>
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    <th>ramachandran_favored</th>
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    <th>ramachandran_outliers</th>
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    <th>Qmean_4</th>
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    <th>Qmean_6</th>
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    <td>0</td>
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    <td>40.65</td>
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    <td>4.06</td>
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    <td>20</td>
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    <td>60</td>
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    <td>-6.45183</td>
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    <td>-5.51397</td>
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===Modeling===
 
 
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1t.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1t.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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                   Figure 1.: 3D Model of H1T peptide Modelled by AlphaFold2.0 displayed in Pyomol
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                   Figure 1.: 3D Model of H1T peptide Modelled by AlphaFold2.0 displayed in Pymol.
  
 
===References===
 
===References===

Latest revision as of 09:06, 13 October 2022


HTRA Binding Peptide 20

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

A reversed sequence of a high affinity binding peptide is proposed for binding to the PDZ domain of HTRA1.

We were motivated by our chosen Tau binding peptide since it was the inverted sequence of a high affinity peptide that shown better affinity, so we decided to try it with our HTRA1 BP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Dry lab characterizations

Modeling

Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0


cbeta_deviations clashscore molprobity ramachandran_favored ramachandran_outliers Qmean_4 Qmean_6
0 40.65 4.06 20 60 -6.45183 -5.51397 



                 Figure 1.: 3D Model of H1T peptide Modelled by AlphaFold2.0 displayed in Pymol.

References

Dammers, C., Yolcu, D., Kukuk, L., Willbold, D., Pickhardt, M., Mandelkow, E., ... & Funke, S. A. (2016). Selection and Characterization of Tau Binding ᴅ-Enantiomeric Peptides with Potential for Therapy of Alzheimer Disease. PLoS One, 11(12), e0167432.