Difference between revisions of "Part:BBa K174009:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | When fusing proteins with CotC, the final sequence should start with CotC and be followed by the sequence of the protein | + | When fusing proteins with CotC, the final sequence should start with CotC and be followed by the sequence of the protein wanted to be localised to the spore coat. |
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+ | Gfp can be added between CotC and the protein wanted to be localised. | ||
===Source=== | ===Source=== |
Latest revision as of 21:32, 11 October 2009
CotC, Bacillus subtilis spore coat protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When fusing proteins with CotC, the final sequence should start with CotC and be followed by the sequence of the protein wanted to be localised to the spore coat.
Gfp can be added between CotC and the protein wanted to be localised.
Source
Bacillus subtilis