Difference between revisions of "Part:BBa K191001:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K191001 short</partinfo>
 
<partinfo>BBa_K191001 short</partinfo>
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===Design Notes===
 
===Design Notes===
No specific problem encountered
+
*LacI promoter was cut with E and S
 
+
*Plasmid with RBS was cut with E and X
 +
*LacI fragment is then ligated into the RBS plasmid.
  
  
 
===Source===
 
===Source===
 
+
<partinfo>BBa_R0010</partinfo> and <partinfo>BBa_B0030</partinfo>
See sources for subparts
+
  
 
===References===
 
===References===

Latest revision as of 12:50, 21 October 2009

Promoter LacI - RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • LacI promoter was cut with E and S
  • Plasmid with RBS was cut with E and X
  • LacI fragment is then ligated into the RBS plasmid.


Source

BBa_R0010 and BBa_B0030

References