Difference between revisions of "Part:BBa K4365020"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Latest revision as of 15:53, 11 October 2022
turboRFP (yeast)
Turbo Red Fluorescent Protein (turboRFP) is an orange fluorescent protein isolated from Entacmaea quadricolor with excitation and emission maxima 553 and 574 respectively.It possesses prolonged fluorescence lifetime and high pH-stability, and is fast-maturing TurboRFP is more than twice brighter than DsRed2 [1].
It could be used as a selection marker or used for protein detection if fused with another protein. This sequence is codon optimized for expression in Saccharomyces cerevisiae.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 683
Usage and Biology
- ↑ Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062