Difference between revisions of "Part:BBa K4509420:Design"

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===Source and Improvement===
 
===Source and Improvement===
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The promoter J23119 is replaced with J23116. This switch in the design will reduce the burden in the cell during overexpression of enzymes
 
  
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===Characterization===
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'''Source:''' Part:[https://parts.igem.org/Part:BBa_K1291071 BBa_K1291071]  HORSERADISH PEROXIDASE TYPE C COMPOSITE PART.
  
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https://static.igem.wiki/teams/4509/wiki/registry/k4509420-graph1.jpg
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The promoter J23119 is replaced with J23116. This switch in the design will reduce the burden on the cell during the overexpression of enzymes.
  
 
===References===
 
===References===
 +
Azevedo, A. M., Martins, V. C., Prazeres, D. M., Vojinovic, V., Cabral, J. M., & Fonseca, L. P. (2003). Horseradish peroxidase: a valuable tool in biotechnology. Biotechnology annual review, 9(3), 1387-2656.
 +
 +
Florea, M., Hagemann, H., Santosa, G., Abbott, J., Micklem, C. N., Spencer-Milnes, X., ... & Chughtai, H. (2016). Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain. Proceedings of the National Academy of Sciences, 113(24), E3431-E3440.
 +
 +
Francisco lucha, FernandoMartínez-García, CarlosLópez-García.1985.A new stabilizing agent for the tetramethyl benzidine (TMB) reaction product in the histochemical detection of horseradish peroxidase (HRP).Journal of Neuroscience Methods.13(2),0165-0270.
 +
 +
Frey, A., Meckelein, B., Externest, D., & Schmidt, M. A. (2000). A stable and highly sensitive 3, 3′, 5, 5′-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. Journal of immunological methods, 233(1-2), 47-56.
 +
 +
Verlander, C. P. (1992). Detection of horseradish peroxidase by colorimetry. Nonisotopic DNA probe techniques, 185-201.
 +
 +
https://parts.igem.org/Part:BBa_K1291071
 +
Albers, S. C., Gallegos, V. A., & Peebles, C. A. M. (2015). Engineering of genetic control tools in Synechocystis sp. PCC 6803 using rational design techniques. Journal of Biotechnology, 216, 36–46.

Latest revision as of 12:17, 12 October 2022


HORSERADISH PEROXIDASE with constitutive promoter J23116


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 565
    Illegal AgeI site found at 719
    Illegal AgeI site found at 1022
  • 1000
    COMPATIBLE WITH RFC[1000]


Source and Improvement

Source: Part:BBa_K1291071 HORSERADISH PEROXIDASE TYPE C COMPOSITE PART.

The promoter J23119 is replaced with J23116. This switch in the design will reduce the burden on the cell during the overexpression of enzymes.

References

Azevedo, A. M., Martins, V. C., Prazeres, D. M., Vojinovic, V., Cabral, J. M., & Fonseca, L. P. (2003). Horseradish peroxidase: a valuable tool in biotechnology. Biotechnology annual review, 9(3), 1387-2656.

Florea, M., Hagemann, H., Santosa, G., Abbott, J., Micklem, C. N., Spencer-Milnes, X., ... & Chughtai, H. (2016). Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain. Proceedings of the National Academy of Sciences, 113(24), E3431-E3440.

Francisco lucha, FernandoMartínez-García, CarlosLópez-García.1985.A new stabilizing agent for the tetramethyl benzidine (TMB) reaction product in the histochemical detection of horseradish peroxidase (HRP).Journal of Neuroscience Methods.13(2),0165-0270.

Frey, A., Meckelein, B., Externest, D., & Schmidt, M. A. (2000). A stable and highly sensitive 3, 3′, 5, 5′-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. Journal of immunological methods, 233(1-2), 47-56.

Verlander, C. P. (1992). Detection of horseradish peroxidase by colorimetry. Nonisotopic DNA probe techniques, 185-201.

https://parts.igem.org/Part:BBa_K1291071 Albers, S. C., Gallegos, V. A., & Peebles, C. A. M. (2015). Engineering of genetic control tools in Synechocystis sp. PCC 6803 using rational design techniques. Journal of Biotechnology, 216, 36–46.