Difference between revisions of "Part:BBa K4252023:Experience"

Latest revision as of 15:30, 11 October 2022

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Applications of BBa_K4252023

We cultured the plasmids transferred into cI857, phlF, R promoter and mCherry at 30℃ ,33℃ ,37℃ and 42℃ . From the fluorescence data corresponding to our temperature gradient, the red fluorescence expression was very weak in the temperature range of 30 ℃ to 34 ℃, indicating that the dimer formed by cI857 inhibited the R promoter at this time, leading to the subsequent weakening of mCherry expression; The strong red fluorescence expression at 37 ℃ to 42 ℃ indicated that cI857 dimer depolymerized and the inhibition of R promoter was relieved, and then mCherry was normally expressed. That is, the temperature sensitive control element cI857 is controlled by temperature

User Reviews

UNIQd1e3da80d7bdcc4b-partinfo-00000000-QINU UNIQd1e3da80d7bdcc4b-partinfo-00000001-QINU

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 ===Applications of BBa_K4252023===
+We cultured the plasmids transferred into cI857, phlF, R promoter and mCherry at 30℃ ,33℃ ,37℃ and 42℃ . From the fluorescence data corresponding to our temperature gradient, the red fluorescence expression was very weak in the temperature range of 30 ℃ to 34 ℃, indicating that the dimer formed by cI857 inhibited the R promoter at this time, leading to the subsequent weakening of mCherry expression; The strong red fluorescence expression at 37 ℃ to 42 ℃ indicated that cI857 dimer depolymerized and the inhibition of R promoter was relieved, and then mCherry was normally expressed.
+That is, the temperature sensitive control element cI857 is controlled by temperature
 ===User Reviews===
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-|-We cultivate BL21 strain with such plasmid at 30 ℃, 33 ℃, 37 ℃ and 42 ℃, test OD600 absorbance value after culture, break the strain and test fluorescence intensity.
+|-
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-Enter the review inofrmation here.
+Enter the review information here.We cultivate BL21 strain with such plasmid at 30 ℃, 33 ℃, 37 ℃ and 42 ℃, test OD600 absorbance value after culture, break the strain and test fluorescence intensity.
 |};
 <!-- End of the user review template -->
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