Difference between revisions of "Part:BBa K4169023"
| (One intermediate revision by the same user not shown) | |||
| Line 5: | Line 5: | ||
This a theophylline-inducible riboswitch which works at the translation level, and the riboswitch produce low levels of background gene expression in the absence of the small molecule theophylline and large (>50-fold) increases in expression in the presence of the ligand. | This a theophylline-inducible riboswitch which works at the translation level, and the riboswitch produce low levels of background gene expression in the absence of the small molecule theophylline and large (>50-fold) increases in expression in the presence of the ligand. | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
<p> | <p> | ||
This is a switch regulated by theophylline at the translation level, that is, a certain concentration of theophylline molecules in the outside world can combine with this riboswitch, and the subsequent translation process of ribosomes can be carried out through conformational changes. | This is a switch regulated by theophylline at the translation level, that is, a certain concentration of theophylline molecules in the outside world can combine with this riboswitch, and the subsequent translation process of ribosomes can be carried out through conformational changes. | ||
</p> | </p> | ||
| + | |||
<!-- --> | <!-- --> | ||
Latest revision as of 14:44, 11 October 2022
a theophylline-inducible riboswitch
This a theophylline-inducible riboswitch which works at the translation level, and the riboswitch produce low levels of background gene expression in the absence of the small molecule theophylline and large (>50-fold) increases in expression in the presence of the ligand.
Usage and Biology
This is a switch regulated by theophylline at the translation level, that is, a certain concentration of theophylline molecules in the outside world can combine with this riboswitch, and the subsequent translation process of ribosomes can be carried out through conformational changes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4
- 1000COMPATIBLE WITH RFC[1000]