Difference between revisions of "Part:BBa K174005"

 
 
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<partinfo>BBa_K174005 short</partinfo>
 
<partinfo>BBa_K174005 short</partinfo>
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This promoter can be induced by xylose and it is regulated by LacI. XylR is normally expressed i B. subtilis and represses the promoter. Addition of xylose will derepress XlyR activity on the promoter.  
 
This promoter can be induced by xylose and it is regulated by LacI. XylR is normally expressed i B. subtilis and represses the promoter. Addition of xylose will derepress XlyR activity on the promoter.  
  
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Biobrick BBa_K143014 is also xylose inducable promoter. They have slightly different operator sites and -10 sequence is one base different. Hence this promoter may have different promoter strength and regulation by XlyR.
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Biobrick BBa_K143014 is also xylose inducable promoter. They have slightly different operator sites and -10 sequence is one base different. Hence, they may have different promoter strength and regulation by XlyR.
  
 
It should be noted that glucose may act as an anti-inducer for xlyR triggering catabolite repression.
 
It should be noted that glucose may act as an anti-inducer for xlyR triggering catabolite repression.
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<partinfo>BBa_K174005 parameters</partinfo>
 
<partinfo>BBa_K174005 parameters</partinfo>
 
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===References===
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#Kim, L., A. Mogk, et al. (1996). "A xylose-inducible Bacillus subtilis integration vector and its application." Gene 181: 71-76.
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#Kreuzer, P., D. Gartner, et al. (1989). "Identification and sequence analysis of the Bacillus subtilis W23 xylR gene and xyl operator." J. Bacteriol. 171(7): 3840-3845.

Latest revision as of 16:49, 10 October 2009

xylA promoter

This promoter can be induced by xylose and it is regulated by LacI. XylR is normally expressed i B. subtilis and represses the promoter. Addition of xylose will derepress XlyR activity on the promoter.

Biobrick BBa_K143014 is also xylose inducable promoter. They have slightly different operator sites and -10 sequence is one base different. Hence, they may have different promoter strength and regulation by XlyR.

It should be noted that glucose may act as an anti-inducer for xlyR triggering catabolite repression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Kim, L., A. Mogk, et al. (1996). "A xylose-inducible Bacillus subtilis integration vector and its application." Gene 181: 71-76.
  2. Kreuzer, P., D. Gartner, et al. (1989). "Identification and sequence analysis of the Bacillus subtilis W23 xylR gene and xyl operator." J. Bacteriol. 171(7): 3840-3845.