Difference between revisions of "Part:BBa K4229035"

Latest revision as of 10:29, 12 October 2022

T2 protein from the full wiffleball of the BMC

The T2 proteins and T3-proteins(BBa_K4229036)assemble and form the second pore structure (together with T1 in the full wiffleball[BBa_K4229048/BBa_K4229049]).

A:Shows the original HO-Shell found in Haliangium orchaceum. B: Shows the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimal wiffleball made just of the H- and T1-protein.

The plasmid with this protein was kindly sent to us by the Kerfeld Group after we found this very interesting paper:

H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next-generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 34
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 34
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 34
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 34
Illegal AgeI site found at 337
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4229035 short</partinfo>
-The T2 proteins builds together with the T3-protein(BBa_K4229036) the second pore structure beside T1 in the full wiffleball(BBa_K4229048/BBa_K4229049).
+The T2 proteins and T3-proteins(BBa_K4229036)assemble and form the second pore structure (together with T1 in the full wiffleball[BBa_K4229048/BBa_K4229049]).
-[[File:BMCs3.jpg|600px|thumb|left|A:Showing the original HO-Shell found in Haliangium orchaceum. B: showing the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimalwiffleball made just our of the H- and T1-protein. ]]
+[[File:BMCs3.jpg|600px|thumb|left| A:Shows the original HO-Shell found in  <i>Haliangium orchaceum</i>. B: Shows the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimal wiffleball made just of the H- and T1-protein. ]]
@@ Line 51: / Line 51: @@
-The plasmid with this protein was sent kindly send to us by the Kerfeld Group after we found this very interesting paper:
+The plasmid with this protein was kindly sent to us by the Kerfeld Group after we found this very interesting paper:
 H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next-generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.