Difference between revisions of "Part:BBa K4129121"
 
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===Usage and Biology===
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=== Fungal synthetic expression system using FunsTF02 ===
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This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF01. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry.
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In this fungal synthetic expression system (SES) FunsTF02 was constitutively expressed from P<i>gpdA</i>. The FunsTF02, which contains a LexA DNA-binding domain, would be able to bind to the LexO binding sites for the minimal promoter fused with LexO binding sites (6xLexO-Pmin). The efficiency of the FunsTF05 activation was measured by the reporter gene mCherry, which is expressed from the 6xLexO-Pmin.
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For characterisation look up BBa_K4129101.
  
 
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Latest revision as of 12:58, 12 October 2022


The fungal synthetic expression system with FunsTF02 and 6xLexO-Pmin


Fungal synthetic expression system using FunsTF02

In this fungal synthetic expression system (SES) FunsTF02 was constitutively expressed from PgpdA. The FunsTF02, which contains a LexA DNA-binding domain, would be able to bind to the LexO binding sites for the minimal promoter fused with LexO binding sites (6xLexO-Pmin). The efficiency of the FunsTF05 activation was measured by the reporter gene mCherry, which is expressed from the 6xLexO-Pmin.

For characterisation look up BBa_K4129101.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4123
    Illegal XbaI site found at 6036
    Illegal PstI site found at 141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
    Illegal NheI site found at 2935
    Illegal PstI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 145
    Illegal BamHI site found at 3461
    Illegal BamHI site found at 5317
    Illegal XhoI site found at 701
    Illegal XhoI site found at 1083
    Illegal XhoI site found at 3610
    Illegal XhoI site found at 3987
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4123
    Illegal XbaI site found at 6036
    Illegal PstI site found at 141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4123
    Illegal XbaI site found at 6036
    Illegal PstI site found at 141
    Illegal NgoMIV site found at 3027
    Illegal NgoMIV site found at 5953
    Illegal AgeI site found at 1290
    Illegal AgeI site found at 1438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1117
    Illegal BsaI.rc site found at 5667
    Illegal SapI site found at 323