Difference between revisions of "File:Level 1 Results TU1 (K4388005).png"

(Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388000, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion react...)
 
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Latest revision as of 20:02, 11 October 2022

Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388000, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.

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current20:02, 11 October 2022Thumbnail for version as of 20:02, 11 October 20221,626 × 902 (885 KB)Tomaslopes (Talk | contribs)BBa_K4388000 replaced by BBa_K4388013 (as the latter correctly contains the lacO site between the promoter and RBS for expression control with IPTG).
13:07, 11 October 2022Thumbnail for version as of 13:07, 11 October 20221,700 × 1,000 (1 MB)Tomaslopes (Talk | contribs)Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388000, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion react...
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