Difference between revisions of "Part:BBa K4317002"

 
 
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Maltose-inducible promoter usable in Bacillus subtilis
 
Maltose-inducible promoter usable in Bacillus subtilis
  
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https://static.igem.org/mediawiki/parts/thumb/f/f8/Pmglv.png/800px-Pmglv.png
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===Usage and Biology===
 
===Usage and Biology===
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https://static.igem.org/mediawiki/parts/thumb/5/51/Promoter_strength.png/800px-Promoter_strength.png
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Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3)
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The mglv proter did not work properly in our Bacillus subtilis expression system. Even without the addition of maltose, mScarlet expression was increased when grown for 24 hours.
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===References===
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Ming, Yang M., et al. "Development of a Bacillus subtilis expression system using the improved Pglv promoter." Microbial cell factories 9.1 (2010): 1-8.
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Song, Yafeng, et al. "High-efficiency secretion of β-mannanase in Bacillus subtilis through protein synthesis and secretion optimization." Journal of agricultural and food chemistry 65.12 (2017): 2540-2548.
  
 
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Latest revision as of 11:21, 11 October 2022


Pmglv

Maltose-inducible promoter usable in Bacillus subtilis

800px-Pmglv.png

Usage and Biology

800px-Promoter_strength.png

Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3)

The mglv proter did not work properly in our Bacillus subtilis expression system. Even without the addition of maltose, mScarlet expression was increased when grown for 24 hours.

References

Ming, Yang M., et al. "Development of a Bacillus subtilis expression system using the improved Pglv promoter." Microbial cell factories 9.1 (2010): 1-8. Song, Yafeng, et al. "High-efficiency secretion of β-mannanase in Bacillus subtilis through protein synthesis and secretion optimization." Journal of agricultural and food chemistry 65.12 (2017): 2540-2548.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal BglII site found at 13
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]