Difference between revisions of "Part:BBa K4252025:Design"

 
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===Design Notes===
 
===Design Notes===
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We find this promoter by reading Professor Chen Guoqiang's article.
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The promoter is regulated by cI857 and inhibited when cI857 forms dimer.
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===Source===
 
===Source===
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===References===
 
===References===
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Wang X, Han JN, Zhang X, Ma YY, Lin Y, Wang H, Li DJ, Zheng TR, Wu FQ, Ye JW, Chen GQ. Reversible thermal regulation for bifunctional dynamic control of gene expression in Escherichia coli. Nat Commun. 2021 Mar 3;12(1):1411. doi: 10.1038/s41467-021-21654-x. PMID: 33658500; PMCID: PMC7930084.

Latest revision as of 15:57, 11 October 2022


R promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter is regulated by cI857 and inhibited when cI857 forms dimer.

Source

gtgcgtgttgactattttacctctggcggtgataatggttgcatgtactaaggaggttg

References

Wang X, Han JN, Zhang X, Ma YY, Lin Y, Wang H, Li DJ, Zheng TR, Wu FQ, Ye JW, Chen GQ. Reversible thermal regulation for bifunctional dynamic control of gene expression in Escherichia coli. Nat Commun. 2021 Mar 3;12(1):1411. doi: 10.1038/s41467-021-21654-x. PMID: 33658500; PMCID: PMC7930084.