Difference between revisions of "Part:BBa K4244034"
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ProC promoter with medium RBS and P1 protospacer in between for dSpyCas9 targeting. | ProC promoter with medium RBS and P1 protospacer in between for dSpyCas9 targeting. | ||
| − | ProC promoter from <partinfo>BBa_K1741012</partinfo> was combined with an RBS derived from RBS calculator. In between these a target region was introduced including PAM and the P1 protospacer | + | ProC promoter from <partinfo>BBa_K1741012</partinfo> was combined with an RBS derived from RBS calculator. In between these a target region was introduced including a PAM and the P1 protospacer, which is targeted by dSpyCas9. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 10:54, 12 October 2022
proC promoter + P1 protospacer + RBS
ProC promoter with medium RBS and P1 protospacer in between for dSpyCas9 targeting.
ProC promoter from BBa_K1741012 was combined with an RBS derived from RBS calculator. In between these a target region was introduced including a PAM and the P1 protospacer, which is targeted by dSpyCas9.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 113
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]