Difference between revisions of "Part:BBa K4361114"
 
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<partinfo>BBa_K4361114 short</partinfo>
 
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This part shows the Blc operator sequence ([[Part:BBa_K4361111]]) after site-directed mutagenesis with primers [[Part:BBa_K4361112]] and [[Part:BBa_K4361113]], resulting in the deletion of the adenine in position 20. This mutation is expected to disrupt BlcR binding to the operator, meaning the protein cannot inhibit downstream gene expression. As such, when inserted in a reporter system such as [[Part:BBa_K4361115]], it may act as a negative control against the wildtype operator sequence.
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This part shows the <i>blc</i> operator sequence after site-directed mutagenesis of the ([[Part:BBa_K4361111]]) with primers [[Part:BBa_K4361112]] and [[Part:BBa_K4361113]], resulting in the deletion of the adenine in position 20.  
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It is known from literature that this mutation disrupts the binding between BlcR and the <i> blc </i> operator sequence [1]. This way BlcR cannot inhibit downstream gene expression.  
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The mutated <i> blc </i> operator sequence is incorperated in a superfolded GFP production plasmid, see [[Part:BBa_K4361115]].
  
 
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<partinfo>BBa_K4361114 parameters</partinfo>
 
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<h3> References </h3>
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[1] Pan, Y., Fiscus, V., Meng, W., Zheng, Z., Zhang, L.-H., Fuqua, C. and Chen, L. (2011). The Agrobacterium tumefaciens Transcription Factor BlcR Is Regulated via Oligomerization. The Journal of Biological Chemistry, [online] 286(23), pp.20431–20440. doi:10.1074/jbc.M110.196154

Latest revision as of 15:44, 12 October 2022


Blc operator sequence SDM deletion

This part shows the blc operator sequence after site-directed mutagenesis of the (Part:BBa_K4361111) with primers Part:BBa_K4361112 and Part:BBa_K4361113, resulting in the deletion of the adenine in position 20.

It is known from literature that this mutation disrupts the binding between BlcR and the blc operator sequence [1]. This way BlcR cannot inhibit downstream gene expression.

The mutated blc operator sequence is incorperated in a superfolded GFP production plasmid, see Part:BBa_K4361115.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Pan, Y., Fiscus, V., Meng, W., Zheng, Z., Zhang, L.-H., Fuqua, C. and Chen, L. (2011). The Agrobacterium tumefaciens Transcription Factor BlcR Is Regulated via Oligomerization. The Journal of Biological Chemistry, [online] 286(23), pp.20431–20440. doi:10.1074/jbc.M110.196154