Difference between revisions of "Part:BBa K4414009"
 
(9 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 +
 
<partinfo>BBa_K4414009 short</partinfo>
 
<partinfo>BBa_K4414009 short</partinfo>
  
−
DNA binding domain
+
==Usage and Biology==
  
−
<!-- Add more about the biology of this part here
+
The Tet repressor protein (TetR) family is a ubiquitous family of transcriptional regulators in a variety of bacteria, with more than 2000 members. The first TetR family identified in Escherichia coli. was Tn10, which controls the expression of genes encoding the tetracycline spillover pump necessary for drug resistance(Ramos et al., 2005). In the absence of tetracycline, TetR binds to the tetracycline operon and inhibits the transcription of tetracycline spillover pump genes. When tetracycline enters the cell, tetracycline binds to TetR and changes its conformation, dissociating TetR from the tet operator (tetO) sequence, thus releasing inhibition. TetR-like transcriptional regulators contain a conserved helix-turn-helix DNA binding region (forming homodimers) and generally act as transcriptional repressors(Das et al., 2016)Their function is to broadly regulate cellular activities (including drug spillover, antibiotic synthesis, amino acid metabolism, etc.). We obtained this DNA by DNA synthesis.This part can be used in level 1 biological laboratory.
−
===Usage and Biology===
+
−
The Tet repressor protein (TetR) family is a ubiquitous family of transcriptional regulators in a variety of bacteria, with more than 2000 members. The first TetR family identified in Escherichia coli. was Tn10, which controls the expression of genes encoding the tetracycline spillover pump necessary for drug resistance.(Ramos et al., 2005) In the absence of tetracycline, TetR binds to the tetracycline operon and inhibits the transcription of tetracycline spillover pump genes. When tetracycline enters the cell, tetracycline binds to TetR and changes its conformation, dissociating TetR from the tet operator (tetO) sequence, thus releasing inhibition. TetR-like transcriptional regulators contain a conserved helix-turn-helix DNA binding region (forming homodimers) and generally act as transcriptional repressors. (Das et al., 2016)Their function is to broadly regulate cellular activities (including drug spillover, antibiotic synthesis, amino acid metabolism, etc.)
+
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===Sequence and Features===
 
<partinfo>BBa_K4414009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4414009 SequenceAndFeatures</partinfo>
  
Line 19: Line 17:
 
<!-- -->
 
<!-- -->
 
===Reference===
 
===Reference===
−
The Tet repressor protein (TetR) family is a ubiquitous family of transcriptional regulators in a variety of bacteria, with more than 2000 members. The first TetR family identified in Escherichia coli. was Tn10, which controls the expression of genes encoding the tetracycline spillover pump necessary for drug resistance.(Ramos et al., 2005) In the absence of tetracycline, TetR binds to the tetracycline operon and inhibits the transcription of tetracycline spillover pump genes. When tetracycline enters the cell, tetracycline binds to TetR and changes its conformation, dissociating TetR from the tet operator (tetO) sequence, thus releasing inhibition. TetR-like transcriptional regulators contain a conserved helix-turn-helix DNA binding region (forming homodimers) and generally act as transcriptional repressors. (Das et al., 2016)Their function is to broadly regulate cellular activities (including drug spillover, antibiotic synthesis, amino acid metabolism, etc.)
+
1. Das, A. T., Tenenbaum, L., & Berkhout, B. (2016). Tet-On Systems For Doxycycline-inducible Gene Expression. Current Gene Therapy, 16(3), 156–167. https://doi.org/10.2174/1566523216666160524144041.
 +
 
 +
2. Ramos, J. L., Martínez-Bueno, M., Molina-Henares, A. J., Terán, W., Watanabe, K., Zhang, X., Gallegos, M. T., Brennan, R., & Tobes, R. (2005). The TetR family of transcriptional repressors. Microbiology and Molecular Biology Reviews: MMBR, 69(2), 326–356. https://doi.org/10.1128/MMBR.69.2.326-356.2005

Latest revision as of 03:51, 14 October 2022


TetR

Usage and Biology

The Tet repressor protein (TetR) family is a ubiquitous family of transcriptional regulators in a variety of bacteria, with more than 2000 members. The first TetR family identified in Escherichia coli. was Tn10, which controls the expression of genes encoding the tetracycline spillover pump necessary for drug resistance(Ramos et al., 2005). In the absence of tetracycline, TetR binds to the tetracycline operon and inhibits the transcription of tetracycline spillover pump genes. When tetracycline enters the cell, tetracycline binds to TetR and changes its conformation, dissociating TetR from the tet operator (tetO) sequence, thus releasing inhibition. TetR-like transcriptional regulators contain a conserved helix-turn-helix DNA binding region (forming homodimers) and generally act as transcriptional repressors(Das et al., 2016)Their function is to broadly regulate cellular activities (including drug spillover, antibiotic synthesis, amino acid metabolism, etc.). We obtained this DNA by DNA synthesis.This part can be used in level 1 biological laboratory.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

1. Das, A. T., Tenenbaum, L., & Berkhout, B. (2016). Tet-On Systems For Doxycycline-inducible Gene Expression. Current Gene Therapy, 16(3), 156–167. https://doi.org/10.2174/1566523216666160524144041.

2. Ramos, J. L., Martínez-Bueno, M., Molina-Henares, A. J., Terán, W., Watanabe, K., Zhang, X., Gallegos, M. T., Brennan, R., & Tobes, R. (2005). The TetR family of transcriptional repressors. Microbiology and Molecular Biology Reviews: MMBR, 69(2), 326–356. https://doi.org/10.1128/MMBR.69.2.326-356.2005