Difference between revisions of "Part:BBa K4361107"

Latest revision as of 13:16, 12 October 2022

pSB1C3, single letter prefix and suffix

This Part shows the linearized DNA sequence of the plasmid Part:pSB1C3 after digestion with EcoRI and PstI, allowing for inserts of BioBricks that have been digested with the same restriction enzymes. Gene can be inserted upfront a T7 promoter, this allows expression in presence of the T7 RNA polymerase. CmR gene ensures the resistance of chloramphenicol.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XhoI site found at 1013
Illegal XhoI site found at 1905
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4361107 short</partinfo>
-This Part shows the linearized DNA sequence of the plasmid [[Part:pSB1C3]] after digestion with EcoRI and PstI, allowing for  inserts of BioBricks that have been digested with the same restriction enzymes (see [[Part:BBa_K4361108]]).
+This Part shows the linearized DNA sequence of the plasmid [[Part:pSB1C3]] after digestion with EcoRI and PstI, allowing for inserts of BioBricks that have been digested with the same restriction enzymes. Gene can be inserted upfront a T7 promoter, this allows expression in presence of the T7 RNA polymerase. <i>CmR</i> gene ensures the resistance of chloramphenicol.
 <!-- Add more about the biology of this part here