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Latest revision as of 08:34, 11 October 2022


pTX2038(Cas9+gRNA+mCherry+ ChlamyHgR)



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1690
    Illegal XbaI site found at 1696
    Illegal XbaI site found at 4614
    Illegal PstI site found at 5138
    Illegal PstI site found at 5211
    Illegal PstI site found at 5864
    Illegal PstI site found at 6410
    Illegal PstI site found at 7832
    Illegal PstI site found at 8036
    Illegal PstI site found at 8066
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1690
    Illegal NheI site found at 5479
    Illegal NheI site found at 13176
    Illegal PstI site found at 5138
    Illegal PstI site found at 5211
    Illegal PstI site found at 5864
    Illegal PstI site found at 6410
    Illegal PstI site found at 7832
    Illegal PstI site found at 8036
    Illegal PstI site found at 8066
    Illegal NotI site found at 4804
    Illegal NotI site found at 11051
    Illegal NotI site found at 12583
    Illegal NotI site found at 13873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1690
    Illegal BglII site found at 4608
    Illegal BglII site found at 5871
    Illegal BglII site found at 6666
    Illegal BglII site found at 7356
    Illegal BglII site found at 9435
    Illegal BamHI site found at 1684
    Illegal BamHI site found at 3498
    Illegal BamHI site found at 9776
    Illegal XhoI site found at 2858
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1690
    Illegal XbaI site found at 1696
    Illegal XbaI site found at 4614
    Illegal PstI site found at 5138
    Illegal PstI site found at 5211
    Illegal PstI site found at 5864
    Illegal PstI site found at 6410
    Illegal PstI site found at 7832
    Illegal PstI site found at 8036
    Illegal PstI site found at 8066
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1690
    Illegal XbaI site found at 1696
    Illegal XbaI site found at 4614
    Illegal PstI site found at 5138
    Illegal PstI site found at 5211
    Illegal PstI site found at 5864
    Illegal PstI site found at 6410
    Illegal PstI site found at 7832
    Illegal PstI site found at 8036
    Illegal PstI site found at 8066
    Illegal NgoMIV site found at 1425
    Illegal NgoMIV site found at 1963
    Illegal NgoMIV site found at 2071
    Illegal NgoMIV site found at 5380
    Illegal NgoMIV site found at 6698
    Illegal NgoMIV site found at 10496
    Illegal NgoMIV site found at 10670
    Illegal AgeI site found at 13670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4727
    Illegal BsaI.rc site found at 4709
    Illegal SapI.rc site found at 9091