Difference between revisions of "Part:BBa K4361105"
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4361105 short</partinfo>
 
<partinfo>BBa_K4361105 short</partinfo>
  
−
Linearized sequence of the pET-11a expression plasmid. This part does not represent the full sequence of pET-11a, but rather the plasmid after digestion with BamHI as to allow for the insertion of a new DNA sequence at the cleavage site (see [[Part:BBa_K4361106]]). The T7 promoter on the plasmid allows for the transcription of the insert when the plasmid is added to a PURE reaction.
+
A pET11a expression system in E. coli is popular because it combines a high protein yield with good regulation over the expression. The T7 RNA polymerase has a lacUV5 promoter that is Isopropyl 𝛃-D-1-thiogalactopyranoside (IPTG) inducible. With the addition of IPTG, the gene upstream of the T7 promoter can be transcribed. <i>AMPR</i> gene ensures the ampicillin resistanceThis part does not represent the full sequence of pET-11a, but rather the plasmid after digestion with BamHI as it allows for the insertion of a new DNA sequence at the cleavage site.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:07, 12 October 2022


pET-11a

A pET11a expression system in E. coli is popular because it combines a high protein yield with good regulation over the expression. The T7 RNA polymerase has a lacUV5 promoter that is Isopropyl 𝛃-D-1-thiogalactopyranoside (IPTG) inducible. With the addition of IPTG, the gene upstream of the T7 promoter can be transcribed. AMPR gene ensures the ampicillin resistanceThis part does not represent the full sequence of pET-11a, but rather the plasmid after digestion with BamHI as it allows for the insertion of a new DNA sequence at the cleavage site.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 316
    Illegal XbaI site found at 5594
    Illegal PstI site found at 1068
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 316
    Illegal NheI site found at 5639
    Illegal PstI site found at 1068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 316
    Illegal BglII site found at 5528
    Illegal BamHI site found at 5672
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 316
    Illegal XbaI site found at 5594
    Illegal PstI site found at 1068
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 316
    Illegal XbaI site found at 5594
    Illegal PstI site found at 1068
    Illegal NgoMIV site found at 3394
    Illegal NgoMIV site found at 3748
    Illegal NgoMIV site found at 3908
    Illegal NgoMIV site found at 5496
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1242
    Illegal SapI.rc site found at 2324