Difference between revisions of "Part:BBa K4297045"
 
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<partinfo>BBa_K4297045 short</partinfo>
 
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plasmid pYLXP' containing the expressiom cassette of alcohol dehydrogenase PAR4
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The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR4, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR2, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 786.58 mg/L. 
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Latest revision as of 03:12, 14 October 2022


PYLXP'-PAR4

The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR4, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR2, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 786.58 mg/L.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
    Illegal NotI site found at 1448
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 249
    Illegal BamHI site found at 4859
    Illegal BamHI site found at 4880
    Illegal XhoI site found at 4316
    Illegal XhoI site found at 4349
    Illegal XhoI site found at 5586
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 950
    Illegal AgeI site found at 4585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1284
    Illegal BsaI site found at 4588
    Illegal BsaI.rc site found at 255
    Illegal BsaI.rc site found at 4582
    Illegal BsaI.rc site found at 5010
    Illegal BsaI.rc site found at 5600