Difference between revisions of "Part:BBa K4317001"
(3 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
− | |||
<partinfo>BBa_K4317001 short</partinfo> | <partinfo>BBa_K4317001 short</partinfo> | ||
P2069 originated from B. pumilus BA06. P2069M, engineered by Cui-Cui Miao and colleagues, has strong promoter activity in Bacillus subtilis. | P2069 originated from B. pumilus BA06. P2069M, engineered by Cui-Cui Miao and colleagues, has strong promoter activity in Bacillus subtilis. | ||
− | + | https://static.igem.org/mediawiki/parts/c/cb/P2069M_ref.png | |
+ | |||
+ | |||
+ | https://static.igem.org/mediawiki/parts/thumb/2/24/P2069M.png/800px-P2069M.png | ||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | https://static.igem.org/mediawiki/parts/thumb/5/51/Promoter_strength.png/800px-Promoter_strength.png | ||
+ | |||
+ | Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3) | ||
+ | |||
+ | ===References=== | ||
+ | Miao, Cui-Cui, et al. "Construction of a high-expression system in Bacillus through transcriptomic profiling and promoter engineering." Microorganisms 8.7 (2020): 1030. | ||
+ | |||
− | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4317001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4317001 SequenceAndFeatures</partinfo> |
Latest revision as of 05:46, 11 October 2022
P2069M
P2069 originated from B. pumilus BA06. P2069M, engineered by Cui-Cui Miao and colleagues, has strong promoter activity in Bacillus subtilis.
Usage and Biology
Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3)
References
Miao, Cui-Cui, et al. "Construction of a high-expression system in Bacillus through transcriptomic profiling and promoter engineering." Microorganisms 8.7 (2020): 1030.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7
Illegal BglII site found at 13
Illegal BamHI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7
- 1000COMPATIBLE WITH RFC[1000]