Difference between revisions of "Part:BBa K4335040"
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 08:37, 11 October 2022
pTX2040(Cas9+gRNA+StayGold+ ChlamyHgR)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1690
Illegal XbaI site found at 1696
Illegal XbaI site found at 4557
Illegal PstI site found at 5081
Illegal PstI site found at 5154
Illegal PstI site found at 5807
Illegal PstI site found at 6353
Illegal PstI site found at 7775
Illegal PstI site found at 7979
Illegal PstI site found at 8009 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1690
Illegal NheI site found at 5422
Illegal NheI site found at 13119
Illegal PstI site found at 5081
Illegal PstI site found at 5154
Illegal PstI site found at 5807
Illegal PstI site found at 6353
Illegal PstI site found at 7775
Illegal PstI site found at 7979
Illegal PstI site found at 8009
Illegal NotI site found at 4747
Illegal NotI site found at 10994
Illegal NotI site found at 12526
Illegal NotI site found at 13816 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1690
Illegal BglII site found at 4551
Illegal BglII site found at 5814
Illegal BglII site found at 6609
Illegal BglII site found at 7299
Illegal BglII site found at 9378
Illegal BamHI site found at 1684
Illegal BamHI site found at 3498
Illegal BamHI site found at 9719
Illegal XhoI site found at 2858 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1690
Illegal XbaI site found at 1696
Illegal XbaI site found at 4557
Illegal PstI site found at 5081
Illegal PstI site found at 5154
Illegal PstI site found at 5807
Illegal PstI site found at 6353
Illegal PstI site found at 7775
Illegal PstI site found at 7979
Illegal PstI site found at 8009 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1690
Illegal XbaI site found at 1696
Illegal XbaI site found at 4557
Illegal PstI site found at 5081
Illegal PstI site found at 5154
Illegal PstI site found at 5807
Illegal PstI site found at 6353
Illegal PstI site found at 7775
Illegal PstI site found at 7979
Illegal PstI site found at 8009
Illegal NgoMIV site found at 1425
Illegal NgoMIV site found at 1963
Illegal NgoMIV site found at 2071
Illegal NgoMIV site found at 3613
Illegal NgoMIV site found at 5323
Illegal NgoMIV site found at 6641
Illegal NgoMIV site found at 10439
Illegal AgeI site found at 13613 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4670
Illegal BsaI.rc site found at 4652
Illegal SapI.rc site found at 9034