Difference between revisions of "Part:BBa K4444004:Design"

Latest revision as of 08:03, 12 October 2022

PhoCl Display GFP

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 52
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 898
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1021
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 60
Illegal BsaI.rc site found at 1543

Design Notes

Modifications to RELEASE:

- TEV cut site is replaced with PhoCl

- Furin cut site is removed

Components:

GFP, tri-transmembrane domain, Phocl , and ER retention motif

Source

RELEASE: https://www.addgene.org/179642/

PhoCl: https://www.addgene.org/164037/

References

Vlahos, Alexander E., et al. “Protease-Controlled Secretion and Display of Intercellular Signals.” Nature Communications, vol. 13, no. 1, 17 Feb. 2022, p. 912, www.nature.com/articles/s41467-022-28623-y, 10.1038/s41467-022-28623-y.

Zhang, W., Lohman, A., Zhuravlova, Y. et al. Optogenetic control with a photocleavable protein, PhoCl. Nat Methods 14, 391–394 (2017). https://doi.org/10.1038/nmeth.4222

@@ Line 7: / Line 7: @@
 ===Design Notes===
-n/a
+<u>Modifications to RELEASE:
+</u> - TEV cut site is replaced with PhoCl
+- Furin cut site is removed
+<u>Components:
+</u>GFP, tri-transmembrane domain, Phocl , and ER retention motif
@@ Line 13: / Line 22: @@
 ===Source===
-Addgene: https://www.addgene.org/179642/
+RELEASE: https://www.addgene.org/179642/
+PhoCl: https://www.addgene.org/164037/
 ===References===
+Vlahos, Alexander E., et al. “Protease-Controlled Secretion and Display of Intercellular Signals.” Nature Communications, vol. 13, no. 1, 17 Feb. 2022, p. 912, www.nature.com/articles/s41467-022-28623-y, 10.1038/s41467-022-28623-y.
+Zhang, W., Lohman, A., Zhuravlova, Y. et al. Optogenetic control with a photocleavable protein, PhoCl. Nat Methods 14, 391–394 (2017). https://doi.org/10.1038/nmeth.4222