Difference between revisions of "Part:BBa K4146103"
 
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<partinfo>BBa_K4146103 short</partinfo>
 
<partinfo>BBa_K4146103 short</partinfo>
  
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CS stands for caffeine synthase, while the TcAncCS1 ancestral gene is derived from Cocobroma, the duplication of TcAncCS1 at node O leads to TcAncCS2, but the kinetic parameters of TcAncCS2 and its progeny enzymes predict that experts will be favored because the 3 methyl Xanthine does not compete for active site binding. We introduced this plasmid gene to facilitate the synthesis of caffeine in engineered strains, thereby regulating the balance of scalp oil.
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TcCS2 is used for caffeine synthesis.
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TcCS2 from C. sinensis has a higher propensity to recognize 3-mX and can convert 3-mX to theobromine.
 
The main caffeine biosynthetic pathway is a sequence consisting of xanthosine→7-methylxanthosine→7-methylxanthine→theobromine→caffeine. Genes encoding N-methyltransferases involved in three of these four reactions have been isolated and the molecular structure of N-methyltransferases investigated.
 
The main caffeine biosynthetic pathway is a sequence consisting of xanthosine→7-methylxanthosine→7-methylxanthine→theobromine→caffeine. Genes encoding N-methyltransferases involved in three of these four reactions have been isolated and the molecular structure of N-methyltransferases investigated.
  
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This gene can be found in E. coli Nissle 1917
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This gene is present in part
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<a href = "https://parts.igem.org/Part:BBa_K4146106"> BBa_K4146106</a>.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 15:21, 11 October 2022


TcCS2

TcCS2 is used for caffeine synthesis. TcCS2 from C. sinensis has a higher propensity to recognize 3-mX and can convert 3-mX to theobromine. The main caffeine biosynthetic pathway is a sequence consisting of xanthosine→7-methylxanthosine→7-methylxanthine→theobromine→caffeine. Genes encoding N-methyltransferases involved in three of these four reactions have been isolated and the molecular structure of N-methyltransferases investigated.


This gene can be found in E. coli Nissle 1917


This gene is present in part <a href = "https://parts.igem.org/Part:BBa_K4146106"> BBa_K4146106</a>.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 549
  • 1000
    COMPATIBLE WITH RFC[1000]