Difference between revisions of "Part:BBa K4245136:Design"
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The Lettuce split aptamer was developed by researchers at the Weill-Cornell Medical College using the SELEX (systematic evolution of ligands by exponential enrichment) process. For the purposes of Lambert iGEM, the sequence complementary to the left portion of the target— the complement of the middle sequence (<partinfo>BBa_K4245131</partinfo>) within the Rolling Circle Product (RCP)— was added to the 3’ end of the Lettuce left sequence. | The Lettuce split aptamer was developed by researchers at the Weill-Cornell Medical College using the SELEX (systematic evolution of ligands by exponential enrichment) process. For the purposes of Lambert iGEM, the sequence complementary to the left portion of the target— the complement of the middle sequence (<partinfo>BBa_K4245131</partinfo>) within the Rolling Circle Product (RCP)— was added to the 3’ end of the Lettuce left sequence. | ||
| − | The experimentation with this version of Lettuce left was not successful, so Lambert iGEM modified the flanking sequence to include one more nucleotide complementary to the target. The modified Lettuce left <partinfo>BBa_K4245134</partinfo> was more functional. | + | The experimentation with this version of Lettuce left was not successful, so Lambert iGEM modified the flanking sequence to include one more nucleotide complementary to the target. The modified Lettuce left (<partinfo>BBa_K4245134</partinfo>) was more functional. |
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 16:39, 13 October 2022
Design Notes
The Lettuce split aptamer was developed by researchers at the Weill-Cornell Medical College using the SELEX (systematic evolution of ligands by exponential enrichment) process. For the purposes of Lambert iGEM, the sequence complementary to the left portion of the target— the complement of the middle sequence (BBa_K4245131) within the Rolling Circle Product (RCP)— was added to the 3’ end of the Lettuce left sequence.
The experimentation with this version of Lettuce left was not successful, so Lambert iGEM modified the flanking sequence to include one more nucleotide complementary to the target. The modified Lettuce left (BBa_K4245134) was more functional.
Source
The part was acquired as DNA oligos from Integrated DNA Technologies.
Lettuce Left
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]