Difference between revisions of "Part:BBa K4129126:Design"
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<partinfo>BBa_K4129126 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4129126 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | The assembly was standardised to assemble the cassettes in bulk. | |
− | + | ||
===Source=== | ===Source=== | ||
− | + | The individual parts were either salvaged from in-house plasmids or <i>de novo</i> synthesised: | |
− | + | <li>BBa_K4129024 was salvaged from a in-house plasmid</li> | |
− | + | <li>FunsTF57 was <i>de novo</i> synthesised from IDT</li> | |
− | + | <li>BBa_K4129115 was salvaged from a in-house plasmid</li> | |
+ | <li>BBa_J06504 salvaged from BBa_K3046004 </li> | ||
+ | <li>BBa_K3669007 was salvaged from a in-house plasmid</li> | ||
+ | <li>BBa_K678036 was salvaged from a in-house plasmid</li> |
Latest revision as of 15:43, 12 October 2022
The fungal synthetic expression system with FunsTF57 and 6xLexO-Pmin
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4186
Illegal XbaI site found at 6099
Illegal PstI site found at 141 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 911
Illegal NheI site found at 2986
Illegal PstI site found at 141 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 145
Illegal BamHI site found at 2920
Illegal BamHI site found at 5380
Illegal XhoI site found at 701
Illegal XhoI site found at 1083
Illegal XhoI site found at 4050 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4186
Illegal XbaI site found at 6099
Illegal PstI site found at 141 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4186
Illegal XbaI site found at 6099
Illegal PstI site found at 141
Illegal NgoMIV site found at 3078
Illegal NgoMIV site found at 6016
Illegal AgeI site found at 1290
Illegal AgeI site found at 1438 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1117
Illegal BsaI.rc site found at 5730
Illegal SapI site found at 323
Design Notes
The assembly was standardised to assemble the cassettes in bulk.
Source
The individual parts were either salvaged from in-house plasmids or de novo synthesised: