Difference between revisions of "Part:BBa K215011"
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This part is OpdA (BBa_K215090) with an N-terminal tag that binds to streptavidin and a C-terminal tag that signals for secretion to the extracellular space (when combined with proper secretion system BBa_K215107). Its expression is IPTG inducible.
 
This part is OpdA (BBa_K215090) with an N-terminal tag that binds to streptavidin and a C-terminal tag that signals for secretion to the extracellular space (when combined with proper secretion system BBa_K215107). Its expression is IPTG inducible.
  
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===Usage and Biology===
 
===Usage and Biology===
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This part (K215011) had not been tested upon submission, but its components have each been individually tested - The enzyme OpdA has been shown to function as predicted ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K215091 K215091]) and it is inserted into an expression vector that has also been shown to work ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K215002 K215002]). 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K215011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K215011 SequenceAndFeatures</partinfo>

Latest revision as of 23:50, 18 October 2009

K215090+K215002: OpdA with Secretion Signal and Streptavidin Binding Tags

This part is OpdA (BBa_K215090) with an N-terminal tag that binds to streptavidin and a C-terminal tag that signals for secretion to the extracellular space (when combined with proper secretion system BBa_K215107). Its expression is IPTG inducible.

Usage and Biology

This part (K215011) had not been tested upon submission, but its components have each been individually tested - The enzyme OpdA has been shown to function as predicted (K215091) and it is inserted into an expression vector that has also been shown to work (K215002).



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 84
    Illegal suffix found in sequence at 1982
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 84
    Illegal SpeI site found at 1983
    Illegal PstI site found at 1997
    Illegal NotI site found at 90
    Illegal NotI site found at 1990
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 84
    Illegal XhoI site found at 1211
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 84
    Illegal suffix found in sequence at 1983
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 84
    Illegal XbaI site found at 99
    Illegal SpeI site found at 1983
    Illegal PstI site found at 1997
    Illegal NgoMIV site found at 308
    Illegal AgeI site found at 503
    Illegal AgeI site found at 842
  • 1000
    COMPATIBLE WITH RFC[1000]