Difference between revisions of "Part:BBa K4129102:Design"
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<partinfo>BBa_K4129102 short</partinfo> | <partinfo>BBa_K4129102 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | FunsTF04 consists of modules from LexA, Hmox1 and B112, and these modules can individually be exchanged with other domains. The exchange of modules follow a standardised USER assembly. | |
===Source=== | ===Source=== | ||
| − | FunsTF04 was <de novo> synthesised as a gene block from IDT | + | FunsTF04 was <i>de novo</i> synthesised as a gene block from IDT |
===References=== | ===References=== | ||
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Radman M. SOS repair hypothesis: phenomenology of an inducible DNA repair which is accompanied by mutagenesis. Basic Life Sci. 1975;5A:355-67. doi: 10.1007/978-1-4684-2895-7_48. PMID: 1103845. | Radman M. SOS repair hypothesis: phenomenology of an inducible DNA repair which is accompanied by mutagenesis. Basic Life Sci. 1975;5A:355-67. doi: 10.1007/978-1-4684-2895-7_48. PMID: 1103845. | ||
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| + | <partinfo>BBa_K4129102 SequenceAndFeatures</partinfo> | ||
Latest revision as of 15:18, 12 October 2022
The fungal synthetic transcription factor, FunsTF04 (LexA-LL-Hmox1-B112-SV40)
Design Notes
FunsTF04 consists of modules from LexA, Hmox1 and B112, and these modules can individually be exchanged with other domains. The exchange of modules follow a standardised USER assembly.
Source
FunsTF04 was de novo synthesised as a gene block from IDT
References
Castaño-Cerezo S, Fournié M, Urban P, Faulon JL, Truan G. Development of a Biosensor for Detection of Benzoic Acid Derivatives in Saccharomyces cerevisiae. Front Bioeng Biotechnol. 2020 Jan 7;7:372. doi: 10.3389/fbioe.2019.00372. PMID: 31970152; PMCID: PMC6959289.
Egland PG, Harwood CS. HbaR, a 4-hydroxybenzoate sensor and FNR-CRP superfamily member, regulates anaerobic 4-hydroxybenzoate degradation by Rhodopseudomonas palustris. J Bacteriol. 2000 Jan;182(1):100-6. doi: 10.1128/JB.182.1.100-106.2000. PMID: 10613868; PMCID: PMC94245
Erill I, Escribano M, Campoy S, Barbé J. In silico analysis reveals substantial variability in the gene contents of the gamma proteobacteria LexA-regulon. Bioinformatics. 2003 Nov 22;19(17):2225-36. doi: 10.1093/bioinformatics/btg303. PMID: 14630651.
Garcia-Bustos J, Heitman J, Hall MN. Nuclear protein localization. Biochim Biophys Acta. 1991 Mar 7;1071(1):83-101. doi: 10.1016/0304-4157(91)90013-m. PMID: 2004116.
Hirai H, Tani T, Kikyo N. Structure and functions of powerful transactivators: VP16, MyoD and FoxA. Int J Dev Biol. 2010;54(11-12):1589-96. doi: 10.1387/ijdb.103194hh. PMID: 21404180; PMCID: PMC3419751.
Ottoz DS, Rudolf F, Stelling J. Inducible, tightly regulated and growth condition-independent transcription factor in Saccharomyces cerevisiae. Nucleic Acids Res. 2014;42(17):e130. doi: 10.1093/nar/gku616. Epub 2014 Jul 17. PMID: 25034689; PMCID: PMC4176152.
Radman M. SOS repair hypothesis: phenomenology of an inducible DNA repair which is accompanied by mutagenesis. Basic Life Sci. 1975;5A:355-67. doi: 10.1007/978-1-4684-2895-7_48. PMID: 1103845.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 945
Illegal BamHI site found at 607
Illegal BamHI site found at 1604
Illegal XhoI site found at 800
Illegal XhoI site found at 1237
Illegal XhoI site found at 1753 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]