Difference between revisions of "Part:BBa K4195179"
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__NOTOC__ | __NOTOC__ | ||
===Biology=== | ===Biology=== | ||
| − | This sequence is a conserved region of toxin gene ''pirA'' | + | This sequence is a conserved region of toxin gene ''pirA''<i>(1)</i>. It’s used as the detection target of RENDR system.<br/> |
'''Ribozyme ENabled Detection of RNA (RENDR)'''<br/> | '''Ribozyme ENabled Detection of RNA (RENDR)'''<br/> | ||
RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the ''Tetrahymena thermophila'' ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.<br/> | RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the ''Tetrahymena thermophila'' ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.<br/> | ||
[[File:T--XMU-China--RENDR.png|400px]]<br/> | [[File:T--XMU-China--RENDR.png|400px]]<br/> | ||
'''Fig. 1 Schematic illustration of RENDR.'''<br/> | '''Fig. 1 Schematic illustration of RENDR.'''<br/> | ||
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===Usage and Design=== | ===Usage and Design=== | ||
This part is used as the target of the RENDR detection system. For toxin pirA, we designed <partinfo>BBa_K4195141</partinfo>, <partinfo>BBa_K4195142</partinfo>, <partinfo>BBa_K4195145</partinfo>, <partinfo>BBa_K4195146</partinfo>, <partinfo>BBa_K4195156</partinfo>, <partinfo>BBa_K4195157</partinfo>, <partinfo>BBa_K4195160</partinfo>, <partinfo>BBa_K4195161</partinfo>, <partinfo>BBa_K4195168</partinfo>, <partinfo>BBa_K4195169</partinfo>, <partinfo>BBa_K4195172</partinfo>, <partinfo>BBa_K4195173</partinfo>. Other related parts are as followings: <partinfo>BBa_K4195151</partinfo>, <partinfo>BBa_K4195183</partinfo>, <partinfo>BBa_K4195184</partinfo>, <partinfo>BBa_K4195185</partinfo>, <partinfo>BBa_K4195186</partinfo>.<br/> | This part is used as the target of the RENDR detection system. For toxin pirA, we designed <partinfo>BBa_K4195141</partinfo>, <partinfo>BBa_K4195142</partinfo>, <partinfo>BBa_K4195145</partinfo>, <partinfo>BBa_K4195146</partinfo>, <partinfo>BBa_K4195156</partinfo>, <partinfo>BBa_K4195157</partinfo>, <partinfo>BBa_K4195160</partinfo>, <partinfo>BBa_K4195161</partinfo>, <partinfo>BBa_K4195168</partinfo>, <partinfo>BBa_K4195169</partinfo>, <partinfo>BBa_K4195172</partinfo>, <partinfo>BBa_K4195173</partinfo>. Other related parts are as followings: <partinfo>BBa_K4195151</partinfo>, <partinfo>BBa_K4195183</partinfo>, <partinfo>BBa_K4195184</partinfo>, <partinfo>BBa_K4195185</partinfo>, <partinfo>BBa_K4195186</partinfo>.<br/> | ||
| − | Reference | + | ====Reference==== |
| − | 1. J. M. S. Lazarte ''et al.'', Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against ''Vibrio parahaemolyticus'' Infection in ''Litopenaeus vannamei'' Shrimp. ''Vaccines (Basel)'' '''9''', (2021). | + | 1. J. M. S. Lazarte ''et al.'', Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against ''Vibrio parahaemolyticus'' Infection in ''Litopenaeus vannamei'' Shrimp. ''Vaccines (Basel)''. '''9''', (2021). |
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| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K4195179 SequenceAndFeatures</partinfo> | ||
Latest revision as of 15:17, 12 October 2022
Biology
This sequence is a conserved region of toxin gene pirA(1). It’s used as the detection target of RENDR system.
Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.
Fig. 1 Schematic illustration of RENDR.
Usage and Design
This part is used as the target of the RENDR detection system. For toxin pirA, we designed BBa_K4195141, BBa_K4195142, BBa_K4195145, BBa_K4195146, BBa_K4195156, BBa_K4195157, BBa_K4195160, BBa_K4195161, BBa_K4195168, BBa_K4195169, BBa_K4195172, BBa_K4195173. Other related parts are as followings: BBa_K4195151, BBa_K4195183, BBa_K4195184, BBa_K4195185, BBa_K4195186.
Reference
1. J. M. S. Lazarte et al., Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp. Vaccines (Basel). 9, (2021).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 205
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]