Difference between revisions of "Part:BBa K4297042"

 
 
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<partinfo>BBa_K4297042 short</partinfo>
 
<partinfo>BBa_K4297042 short</partinfo>
  
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plasmid pYLXP' containing the expressiom cassette of alcohol dehydrogenase PARL
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The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR1, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde.  The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 762.71 mg/L.
  
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        <img src="https://static.igem.wiki/teams/4297/wiki/par1.jpg" width="50%" style="float:center">
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:10, 14 October 2022


PYLXP'-PAR1

The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR1, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 762.71 mg/L.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
    Illegal NheI site found at 5520
    Illegal NotI site found at 1448
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 249
    Illegal XhoI site found at 4316
    Illegal XhoI site found at 4349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4638
    Illegal NgoMIV site found at 4668
    Illegal NgoMIV site found at 4901
    Illegal NgoMIV site found at 5133
    Illegal NgoMIV site found at 5304
    Illegal AgeI site found at 950
    Illegal AgeI site found at 4585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1284
    Illegal BsaI site found at 4588
    Illegal BsaI.rc site found at 255
    Illegal BsaI.rc site found at 4582
    Illegal BsaI.rc site found at 4767
    Illegal BsaI.rc site found at 5621
    Illegal SapI.rc site found at 5042