Difference between revisions of "Part:BBa K4195119"
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<partinfo>BBa_K4195119 short</partinfo>
 
<partinfo>BBa_K4195119 short</partinfo>
  
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I0500 is the inducible pBad/araC promoter. This composite part can express GFP induced by arabinose, which is used to characterize the function of I0500.
 
===Biology and Usage===
 
===Biology and Usage===
 
I0500 is the inducible pBad/araC promoter. The promoter of the araBAD operon from <i>E. coli</i> is activated in the presence of arabinose and the absence of glucose and directs expression of genes encoding enzymes required for arabinose metabolism.
 
I0500 is the inducible pBad/araC promoter. The promoter of the araBAD operon from <i>E. coli</i> is activated in the presence of arabinose and the absence of glucose and directs expression of genes encoding enzymes required for arabinose metabolism.
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<i>gfp</i> can express green fluorescence protein. The expression level of GFP can be used to characterize the function of I0500.
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<i>Gfp</i> can express green fluorescence protein. The expression level of GFP can be used to characterize the function of I0500.
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===Characterization===
 
===Characterization===
 
Agarose Gel Electrophoresis When we were constructing this circuit, regular PCR was used to certify the plasmid was correct. We got the target (2360 bp).  
 
Agarose Gel Electrophoresis When we were constructing this circuit, regular PCR was used to certify the plasmid was correct. We got the target (2360 bp).  
  
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[[File:T--XMU-China--BBa K4195119.png|200px]]
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'''<b>Fig 1. The result of colony PCR. Plasmid pSB1C3. </b>'''
  
  

Latest revision as of 15:36, 12 October 2022


I0500-B0034-GFP-B0015

I0500 is the inducible pBad/araC promoter. This composite part can express GFP induced by arabinose, which is used to characterize the function of I0500.

Biology and Usage

I0500 is the inducible pBad/araC promoter. The promoter of the araBAD operon from E. coli is activated in the presence of arabinose and the absence of glucose and directs expression of genes encoding enzymes required for arabinose metabolism. Gfp can express green fluorescence protein. The expression level of GFP can be used to characterize the function of I0500.

Characterization

Agarose Gel Electrophoresis When we were constructing this circuit, regular PCR was used to certify the plasmid was correct. We got the target (2360 bp).

T--XMU-China--BBa K4195119.png

Fig 1. The result of colony PCR. Plasmid pSB1C3.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1880
    Illegal SapI site found at 961