Difference between revisions of "Part:BBa K4343113"

Latest revision as of 14:16, 10 October 2022

Sc4Des

Sc4Des encodes Δ-4 desaturase from Schizochytrium sp. which functions in the Δ-9 elongase/desaturase pathway and could convert C22:5 to C22:6.

Description

The Sc4Des gene encodes a ∆-4 desaturase of 427 amino acids from Schizochytrium sp. that catalyzes the conversion of Docosapentaenoic acid (DPA; C22:5n-3) to Docosahexaenoic acid (DHA; C22:6n- 3). (Fig: The Gel Electrophoretic Image of Sc4Des gene)

Literature

Some studies have shown that Δ-4 desaturase from Schizochytrium sp.is used to produce Docosahexaenoic acid (DHA).

Reference

Ren, L. J., Zhuang, X. Y., Chen, S. L., Ji, X. J., & Huang, H. (2015). Introduction of ω-3 desaturase obviously changed the fatty acid profile and sterol content of Schizochytrium sp. Journal of agricultural and food chemistry, 63(44), 9770-9776.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 356
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI site found at 664
Illegal BsaI site found at 813
Illegal BsaI site found at 846
Illegal BsaI.rc site found at 1301

@@ Line 7: / Line 7: @@
 ===Description===
-The Sc4Des gene encodes a ∆-4 desaturase of 427 amino acids from Schizochytrium sp. that catalyzes the conversion of Docosapentaenoic acid (DPA; C22:5n-3) to Docosahexaenoic acid (DHA; C22:6n- 3).（Fig. The Gel Electrophoretic Image of Sc4Des gene）
+The Sc4Des gene encodes a ∆-4 desaturase of 427 amino acids from Schizochytrium sp. that catalyzes the conversion of Docosapentaenoic acid (DPA; C22:5n-3) to Docosahexaenoic acid (DHA; C22:6n- 3). (Fig: The Gel Electrophoretic Image of Sc4Des gene)
 <center>https://static.igem.wiki/teams/4343/wiki/sc4des.png</center>