Difference between revisions of "Part:BBa K4461512:Experience"

 
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===Applications of BBa_K4461512===
 
===Applications of BBa_K4461512===
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We used enzyme digestion and ligation to ligate three devices and pSB1C3. To check if we insert successfully, we use those primers used to add suffix and prefix for them to clone three promoters respectively. The nirBDC promoter with suffix and prefix should be 357bp, and the glpABC promoter and hchA promoter should be 312bp and 261bp.
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[[File:Ligation promoter test.jpg|300px|thumb|center|Fig1. The result shows that three promoters are in this construct.]]
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Then, the FP intensity and OD are measured three times, Fig.2 shows the result.
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[[File:Ligation FP+OD test.png|300px|thumb|center|Fig.2]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 17:19, 10 October 2022


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4461512

We used enzyme digestion and ligation to ligate three devices and pSB1C3. To check if we insert successfully, we use those primers used to add suffix and prefix for them to clone three promoters respectively. The nirBDC promoter with suffix and prefix should be 357bp, and the glpABC promoter and hchA promoter should be 312bp and 261bp.

Fig1. The result shows that three promoters are in this construct.

Then, the FP intensity and OD are measured three times, Fig.2 shows the result.

Fig.2

User Reviews

UNIQc27e5b5d971e8f80-partinfo-00000000-QINU UNIQc27e5b5d971e8f80-partinfo-00000001-QINU