Difference between revisions of "Part:BBa K215260"

Latest revision as of 19:07, 1 October 2009

Lpp + 1tmr OmpA + GS Linker + TEV site + WT Streptavidin + 6His

This is a fusion protein that combines Lpp, OmpA, and WT streptavidin for the purpose of displaying streptavidin on the surface of the cell. Lpp sequence targets protein to the periplasm, and OmpA transmembrane helix anchors it into the outer membrane with WT strepavidin on the extracellular side of the outer membrane. TEV protease site included for easily removal of strepavidin (and any other proteins bound to streptavidin) by treatment with TEV protease.

See BBa_K215210 for the full inducible WT streptavidin display system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 256
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 298
Illegal AgeI site found at 349
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K215260 short</partinfo>
-This is a fusion protein that combines Lpp, OmpA, and WT streptavidin.
+This is a fusion protein that combines Lpp, OmpA, and WT streptavidin for the purpose of displaying streptavidin on the surface of the cell.  Lpp sequence targets protein to the periplasm, and OmpA transmembrane helix anchors it into the outer membrane with WT strepavidin on the extracellular side of the outer membrane.  TEV protease site included for easily removal of strepavidin (and any other proteins bound to streptavidin) by treatment with TEV protease.
-See BBa_K215210 for the full induceable WT streptavidin dispaly system.
+See BBa_K215210 for the full inducible WT streptavidin display system.
 <!-- Add more about the biology of this part here