Difference between revisions of "Part:BBa K4388006:Experience"
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===Applications of BBa_K4388006=== | ===Applications of BBa_K4388006=== | ||
| + | <h2>KCL iGEM 2022</h2> | ||
| + | <p>Through JUMP type IIS Level 1, this transcriptional unit (BBa_K4388006) was assembled in a one-pot digestion reaction with BsaI and ligation into pJUMP29-1B. Successful ligation and transformation were selected for by identifying non-fluorescent colonies on kanamycin agar plates (Fig. 1).</p> | ||
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| + | [[File:TU2 resized.png]] | ||
| + | <p>Figure 1. Construction of Transcriptional Unit (TU) BBa_K4388006 into Level 1 pJUMP29-1B vector. Level 0 parts BBa_K4388011, BBa_K4388002, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388006 in pJUMP29-1B via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.</p> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 19:35, 11 October 2022
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Applications of BBa_K4388006
KCL iGEM 2022
Through JUMP type IIS Level 1, this transcriptional unit (BBa_K4388006) was assembled in a one-pot digestion reaction with BsaI and ligation into pJUMP29-1B. Successful ligation and transformation were selected for by identifying non-fluorescent colonies on kanamycin agar plates (Fig. 1).
Figure 1. Construction of Transcriptional Unit (TU) BBa_K4388006 into Level 1 pJUMP29-1B vector. Level 0 parts BBa_K4388011, BBa_K4388002, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388006 in pJUMP29-1B via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.
User Reviews
UNIQf01387c561186e2d-partinfo-00000000-QINU UNIQf01387c561186e2d-partinfo-00000001-QINU