Difference between revisions of "Part:BBa K4245204"

Latest revision as of 21:33, 11 October 2022

hsa-mir-133a-3p RCA Padlock Probe

This part is the sequence for the hsa-miR-133a-3p RCA Padlock Probe, which our team designed to be specific to BBa_K4245009, hsa-miR-133a-3p. This miRNA acts as a biomarker for Coronary Artery Disease, and is therefore potentially useful for the early detection of this condition. When using the Rolling Circle Amplification approach, the produced transcript contains repeats of the middle sequence (to which the DNA fluorescent aptamer split lettuce (BBa_K4245134/ BBa_K4245135) and the FAM and BHQ1 labeled linear probes (BBa_K4245130/BBa_K4245132) bind) and the 3’ (BBa_K4245103) and 5’(BBa_K4245110) arms of the padlock probe.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4245204 short</partinfo>
+<br>
-hsa-mir-133a-3p RCA Padlock Probe
+<br>
+This part is the sequence for the hsa-miR-133a-3p RCA Padlock Probe, which our team designed to be specific to <partinfo>BBa_K4245009</partinfo>, hsa-miR-133a-3p. This miRNA acts as a biomarker for Coronary Artery Disease, and is therefore potentially useful for the early detection of this condition. When using the Rolling Circle Amplification approach, the produced transcript contains repeats of the middle sequence (to which the DNA fluorescent aptamer split lettuce (<partinfo>BBa_K4245134</partinfo>/ <partinfo>BBa_K4245135</partinfo>) and the FAM and BHQ1 labeled linear probes (<partinfo>BBa_K4245130</partinfo>/<partinfo>BBa_K4245132</partinfo>) bind) and the 3’ (<partinfo>BBa_K4245103</partinfo>) and 5’(<partinfo>BBa_K4245110</partinfo>) arms of the padlock probe.
+<br>
+<br>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===