Difference between revisions of "Part:BBa K4497002"

 
 
(4 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4497002 short</partinfo>
 
<partinfo>BBa_K4497002 short</partinfo>
  
−
.
+
Anti-GFP nanobody is a nanobody directed against GFP [1].
 +
 
 +
 
 +
When using this part consider:
 +
 
 +
Add Signaling Sequence, there is no Start Codon, there is no Stop Codon
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 13: Line 18:
  
  
 +
===References===
 +
 +
[1]: Zhang, Z., Wang, Y., Ding, Y., & Hattori, M. (2020). Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification. Scientific Reports, 10(1), 1–10. https://doi.org/10.1038/s41598-020-62606-7
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4497002 parameters</partinfo>
 
<partinfo>BBa_K4497002 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 11:56, 13 October 2022


Anti-GFP nanobody

Anti-GFP nanobody is a nanobody directed against GFP [1].


When using this part consider:

Add Signaling Sequence, there is no Start Codon, there is no Stop Codon

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 241
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 241
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 241
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]: Zhang, Z., Wang, Y., Ding, Y., & Hattori, M. (2020). Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification. Scientific Reports, 10(1), 1–10. https://doi.org/10.1038/s41598-020-62606-7