Difference between revisions of "Part:BBa K4165020"
 
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<partinfo>BBa_K4165020 short</partinfo>
 
<partinfo>BBa_K4165020 short</partinfo>
  
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This part encodes for 6x Histidine tag used for purification and characterization of proteins
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This part encodes 6x Histidine tag used for purification and characterization of proteins
  
 
===Usage and Biology===
 
===Usage and Biology===
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His tag is used for purifying and detecting the recombinant protein without a protein-specific antibody or probe (1). Parts of our systems are fused with his tag for purification and pull-down assay.
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His tag is used for purifying and detecting the recombinant protein without a protein-specific antibody or probe <sup>[1]</sup>. Parts of our systems are fused with his tag for purification and pull-down assay.
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===References===
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1- Spriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B. Purification of His-Tagged Proteins. Methods Enzymol. 2015;559:1-15. doi: 10.1016/bs.mie.2014.11.003. Epub 2015 May 4. PMID: 26096499.
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<span class='h3bb'>Sequence and Features</span>
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===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K4165020 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165020 SequenceAndFeatures</partinfo>
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===References===
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1- Spriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B. Purification of His-Tagged Proteins. Methods Enzymol. 2015;559:1-15. doi: 10.1016/bs.mie.2014.11.003. Epub 2015 May 4. PMID: 26096499.
  
  

Latest revision as of 13:30, 10 October 2022


6x Histidine tag

This part encodes 6x Histidine tag used for purification and characterization of proteins

Usage and Biology

His tag is used for purifying and detecting the recombinant protein without a protein-specific antibody or probe [1]. Parts of our systems are fused with his tag for purification and pull-down assay.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1- Spriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B. Purification of His-Tagged Proteins. Methods Enzymol. 2015;559:1-15. doi: 10.1016/bs.mie.2014.11.003. Epub 2015 May 4. PMID: 26096499.