Difference between revisions of "Part:BBa K4497000"
Line 3: | Line 3: | ||
<partinfo>BBa_K4497000 short</partinfo> | <partinfo>BBa_K4497000 short</partinfo> | ||
− | . | + | This linker sequence of 25 amino acids (EAAAK)5 has pH-sensitive auto-catalytic according to Wu et al. |
+ | |||
+ | Reference: | ||
+ | Wu, Y. J., Fan, C. Y., & Li, Y. K. (2009). Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877(31), 4015–4021. https://doi.org/10.1016/J.JCHROMB.2009.10.009 | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 14:59, 12 October 2022
pH-sensitive protein linker (EAAAK)5
This linker sequence of 25 amino acids (EAAAK)5 has pH-sensitive auto-catalytic according to Wu et al.
Reference: Wu, Y. J., Fan, C. Y., & Li, Y. K. (2009). Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877(31), 4015–4021. https://doi.org/10.1016/J.JCHROMB.2009.10.009
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]