Difference between revisions of "Part:BBa K200006"
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Sequence codes for trehalose phosphatase enzyme. This enzyme is the second of two required for the conversion of glucose to [http://en.wikipedia.org/wiki/Trehalose trehalose]. <br> | Sequence codes for trehalose phosphatase enzyme. This enzyme is the second of two required for the conversion of glucose to [http://en.wikipedia.org/wiki/Trehalose trehalose]. <br> | ||
| − | This enzyme catalyses the following reaction: <br> | + | This enzyme catalyses the following reaction: <br><br><br> |
| − | + | ||
| − | <br> | + | |
| + | [[Image:II09_OtsB.png]] | ||
| + | <br><br><br> | ||
Trehalose is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock <cite>otsb1</cite>, and is naturally produced in Escherichia Coli. | Trehalose is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock <cite>otsb1</cite>, and is naturally produced in Escherichia Coli. | ||
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OtsA mutation will block the synthesis of the trehalose-6-phosphase synthase, which is the enzyme that converts glucose-6-phosphate to trehalose-6-phosphate. <cite>otsb2</cite> | OtsA mutation will block the synthesis of the trehalose-6-phosphase synthase, which is the enzyme that converts glucose-6-phosphate to trehalose-6-phosphate. <cite>otsb2</cite> | ||
| − | + | <br><br>The gene was used alongside [[Part:BBa_K200005 |OtsA]] by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] team as part of the storage protection mechanism. It was also previously used by the UC Berkeley 2007 iGEM team as part of the [http://2007.igem.org/Berkeley_UC Bactoblood] project to enable freeze-drying of the system. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 15:44, 29 September 2009
OtsB: Part 2 of 2 coding for trehalose producing enzymes
Sequence codes for trehalose phosphatase enzyme. This enzyme is the second of two required for the conversion of glucose to [http://en.wikipedia.org/wiki/Trehalose trehalose].
This enzyme catalyses the following reaction:
Trehalose is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock otsb1, and is naturally produced in Escherichia Coli.
We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.
Usage and Biology
Transcription of OtsB gene is activated by osmotic stress in E. coli.otsb1
mRNA of OtsB is more stable at 16°C, therefore, it is a cold inducible mRNA.otsb2
OtsA mutation will block the synthesis of the trehalose-6-phosphase synthase, which is the enzyme that converts glucose-6-phosphate to trehalose-6-phosphate. otsb2
The gene was used alongside OtsA by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] team as part of the storage protection mechanism. It was also previously used by the UC Berkeley 2007 iGEM team as part of the [http://2007.igem.org/Berkeley_UC Bactoblood] project to enable freeze-drying of the system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 455
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
References
<biblio>
- otsb1 pmid=12105274
- otsb2 pmid=1310094
</biblio>