Difference between revisions of "Part:BBa K4175007"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K4175007 short</partinfo> | <partinfo>BBa_K4175007 short</partinfo> | ||
+ | |||
+ | In our project, this promoter is used for IL-6_scfv-Notch-Gal4KRAB (<partinfo>BBa_K4175008</partinfo>), IL-6R-Notch-Gal4KRAB (<partinfo>BBa_K4175010</partinfo>) and IL-6R-PD-1 (<partinfo>BBa_K4175012</partinfo>) expression. | ||
===Sequence and Features=== | ===Sequence and Features=== |
Latest revision as of 10:30, 10 October 2022
MND promoter
In our project, this promoter is used for IL-6_scfv-Notch-Gal4KRAB (BBa_K4175008), IL-6R-Notch-Gal4KRAB (BBa_K4175010) and IL-6R-PD-1 (BBa_K4175012) expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 60
Illegal XbaI site found at 271
Illegal PstI site found at 55 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 60
Illegal PstI site found at 55 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 60
Illegal BamHI site found at 76
Illegal XhoI site found at 19 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 60
Illegal XbaI site found at 271
Illegal PstI site found at 55 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 60
Illegal XbaI site found at 271
Illegal PstI site found at 55 - 1000COMPATIBLE WITH RFC[1000]